In silico Identification of Novel Toxin Homologs and Associated Mobile Genetic Elements in Clostridium perfringens
نویسندگان
چکیده
منابع مشابه
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B
Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...
متن کاملClostridium Perfringens Toxin Types Associated with Meat: Review in Iran
Food poisoning due to Clostridium perfringens (C. perfringens) is a major food health problem, particularly in terms of meat consumption. Due to human’s susceptibility to this pathogen, detection methods and prevention measures should be implemented to reduce its incidence. Several pathogenic strains of C. perfringens have been identified so far. One of the potential concerns about this bacteri...
متن کاملCloning of Clostridium perfringens iota toxin gene in Escherichia coli
Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...
متن کاملIdentification of enterototxin harboring gene among Clostridium perfringens isolates with different toxin types in Iran
Background: Clostridium perfringens is known as the most widely distributed pathogenic microorganism in nature. It is an extremely important pathogen of human and domestic animals. In a commonly used classification scheme, C. perfringens is divided into five toxinotypes (A to E) based on the production of four major toxins (alpha, beta, epsilon, and iota). Enterotoxin is not usually used for C....
متن کاملIdentification of a novel locus that regulates expression of toxin genes in Clostridium perfringens.
A novel gene that regulates the alpha-toxin (plc), kappa-toxin (colA), and theta;-toxin (pfoA) genes was identified using toxin-negative mutant strains of Clostridium perfringens. The cloned 3.2-kb fragment contained the virX gene encoding a 51-amino acid polypeptide of unknown function that seemed to be responsible for the activation of toxin genes. The virX knock out mutant of wild-type strai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pathogens
سال: 2019
ISSN: 2076-0817
DOI: 10.3390/pathogens8010016